Mushroom extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line
Conference Paper
Overview
Overview
abstract
Mushrooms are part of the sexual life cycle of particular fungi with specific metabolic
pathways, and therefore may contain a largely unexploited source of powerful new pharmaceutical products with potential antitumor properties [1,2]. Furthermore, they
may have potential as functional foods. Suillus collinitus is an edible mushroom found
in European pine forests. The aim of this work was to study the cytotoxic potential of
extracts from this mushroom in various cancer cell lines.
Different extracts (methanolic, ethanolic and aqueous) were prepared and extractinduced
cell growth inhibition was assessed with the sulforhodamine B assay in four
human tumour cell lines (lung, breast, colon and gastric cancer). The methanolic
extract was further characterized in its phenolic composition by HPLC-DAD. The
effects of the extract on cell cycle profile and apoptosis were evaluated by flow
cytometry and the effect on the expression levels of proteins related to cell cycle and
apoptosis was further investigated by Western blotting.
Regarding cell growth inhibition, the methanolic extract was the most potent one,
particularly in MCF-7 cells (GI50 25.2±0.2lg/ml). Moreover, the GI50 concentration
ninduced a G1 cell cycle arrest, with a concomitant decrease in the percentage of cells in
the S phase. Furthermore, it caused an increase in the percentage of apoptotic cells,
from 6.0±0.2% in untreated cells, to 15.3±2.0% in treated cells. In addition, 48h
treatment with the GI50 concentration caused a strong increase in the levels of p53, p21,
cleaved caspase-3 and cleaved PARP, together with a decrease in Bcl-2. The main
components identified in the methanolic extract were: protocatechuic acid
(5.2±0.2mg/kg dw), p-hydroxybenzoic acid (14.1±1.2mg/kg) and cinnamic acid
(1.3±0.2mg/kg).
Results indicate that Suillus colinitus is a promising source of bioactive compounds.
Particularly, its methanolic extract appears to have a p53-mediated effect on the
normal cell cycle distribution and apoptosis induction in human breast tumor cells.
