Ganoderma lucidum is one of the most widely studied mushroom species, particularly
in what concerns its medicinal properties. Previous studies (including those from some of
us) have shown some evidence that the methanolic extract of G. lucidum affects cellular
autophagy. However, it was not known if it induces autophagy or decreases the autophagic
flux. The treatment of a gastric adenocarcinoma cell line (AGS) with the mushroom extract
increased the formation of autophagosomes (vacuoles typical from autophagy). Moreover,
the cellular levels of LC3-II were also increased, and the cellular levels of p62 decreased,
confirming that the extract affects cellular autophagy. Treating the cells with the extract together with lysossomal protease inhibitors, the cellular levels of LC3-II and p62 increased.
The results obtained proved that, in AGS cells, the methanolic extract of G. lucidum causes
an induction of autophagy, rather than a reduction in the autophagic flux. To our knowledge,
this is the first study proving that statement.
IPATIMUP integrates the i3S Research Unit, which is partially supported by FCT, the Portuguese
Foundation for Science and Technology. This work is funded by FEDER funds through the Operational
Programme for Competitiveness Factors-COMPETE and National Funds through the FCT-Foundation for
Science and Technology, under the projects “PEst-C/SAU/LA0003/2013”, NORTE-07-0162-FEDER-
00018-Contributos para o reforço da capacidade do IPATIMUP enquanto actor do sistema regional de
inovação and NORTE-07-0162-FEDER-000067-Reforço e consolidação da capacidade infraestrutural
do IPATIMUP para o sistema regional de inovação, both supported by Programa Operacional Regional
do Norte (ON.2-O Novo Norte), through FEDER funds under the Quadro de Referência Estratégico
Nacional (QREN). The authors thank COMPETE/QREN/EU for the financial support to CIMO (strategic
project PEst-OE/AGR/UI0690/2014) and L. Barros. Authors also thank Foundation for Science and
Technology (FCT, Portugal) for R.T. Lima grant (SFRH/BPD/68787/2010) and QREN for the grant of
F.S. Reis (NORTE-07-0124-FEDER-000023).
