Effect of temperature on the bioactive properties of bee pollen
Conference Paper
Overview
Overview
abstract
Bee pollen is flower pollen collected by the honey bee, Apis mellifera, for the purpose
of feeding its larvae in the early stages of development. lt is recognized to be a valuable
apitherapeutic product with potential for medical, health and nutritional applications.
The objective of this work was to compares the effect of different storage conditions in
the bioactive compounds and biological properties of bee pollen.
Bee pollen is flower pollen collected by the honey bee, Apis mellifera, for the purpose of feeding its larvae in the early stages of development. It is recognized to be a valuable apitherapeutic product with potential for medical, health and nutritional applications.
The objective of this work was to compares the effect of different storage conditions in the bioactive compounds and biological properties of bee pollen.
The amount in phenolics compounds determined by the method of Folin-Ciocalteu varied from 32.64 to 48.40 mg GAE/g, for the dehydrated and refrigerated samples, respectively. Significant differences were verified among the two conservation processes. the amount in total flavonoids determined by the method chloride of aluminum were not verified among significant differences in the samples (6.58 mg CAE/g) and (6.99 mg CAE/g).Concerning the amount of total flavonoids determined by the method of aluminum chloride were not significant differences between the dehydrated (6.58 mg of DEA / g) and refrigerated samples (CAE 6.99 mg / g).
The antioxidant capacity of BP extracts was assessed through the scavenging effect on DPPH (2.2-diphenyl-1-picrylhydrazyl) and reducing power. The concentration-dependent antioxidative capacity was verified in DPPH and reducing power assays. Low values of EC50 on DPPH scavenging assay were obtained for fresh and dehydrated samples 0.74 and 1.16 mg/mL, respectively. For reducing power the values obtained for the refrigerated samples and dehydrated were similar. The high activity of refrigerated samples could be related with the conservation process. Regarding to phenolics compounds determined by HPLC method, were identified four (4) families, flavones being the most abundant one in the refrigerated and dehydrated samples.
We also verified that the presence of pollen differentially affected the growth of bacterium Gram-positive (Staphylococcus xylosus, Staphylococcus epidermidis), Gram-negative (Shigella spp, Klebsiella pneumoniae) and yeasts (Cândida parapilosis, Cândida menbranifaciens, Cândida gllabrata) under study, depending this on the microorganism and the method of BP conservation.
