Phenolic profile obtained by HPLC-DAD-ESI/MS and in vitro bioactivities of Equisetum giganteum L. and Tilia platyphyllos Scop.
Conference Paper
Overview
Overview
abstract
Medicinal plants are a source of a wide range of bioactive compounds, such as alkaloids, terpenes,
steroids and phenolic compounds, which are responsible for multiple biological effects [1, 2]. In the
present work, the phenolic composition and bioactive potential of the aqueous and hydroethanolic
extracts of Equisetum giganteum L. and Tilia platyphyllos Scop. were evaluated. The phenolic
compounds were determined using a Hewlett-Packard 1100 chromatograph, with a diode array detector
coupled to a MS detector API 3200 Qtrap through an ESI source and a triple quadrupole-ion trap mass
analyser, while the bioactive properties were evaluated in terms of antioxidant, anti-inflammatory, and
cytotoxic activities. The hydroethanolic extracts revealed higher amounts of phenolic compounds than
infusions, being the concentration of flavonoids (81% of the phenolic composition) remarkably higher than
the phenolic acids content (19%), in both species and extracts. T. platyphyllos presented a higher
phenolic content (50.4 ± 0.4 mg/g of hydroethanolic extract and 11.65 ± 0.05 mg/g of lyophilized
infusion), than E. giganteum (21.7 ± 0.4 mg/g and 4.98 ± 0.03 mg/g, respectively). Moreover, kaempferol-
O-glucoside-O-rutinoside was the most abundant flavonoid in E. giganteum extract, while protocatechuic
acid and (−)-epicatechin were the most abundant phenolic acid and flavonoid, respectively, in T.
platyphyllos extract. Regarding the bioactive assays, both extracts obtained from T. platyphyllos showed
the highest potential and none of the extracts showed toxicity in non-tumor liver cells. These biological
properties were highly correlated with its content and composition in phenolic compounds. Thus, it would
be interesting to evaluate the in vivo efficacy of both plant extracts to unveil the involved modes of action
and to establish effective therapeutic doses.
The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme
PT2020 for financial support to CIMO (UID/AGR/00690/2013) and L. Barros contract; to the Interreg España-Portugal
for financial support through the project 0377_Iberphenol_6_E.
