Chemical characterization and antioxidant activity of three mushroom species from Poland
Conference Paper
Overview
Overview
abstract
Mushrooms contain a huge diversity of biomolecules with nutritional [1) and/or bioactive
properties [2]. Phenolic compounds, ascorbic acid and tocopherols are considered to
be the most responsible for their antioxidant activity [3). In the present work, Boletus
edulis, Lentinus edodes and Xerocomus badius. three mushroom species originated
from Poland were analyzed for their chemical composition (nutritional value, primary
and secondary metabolites) and antioxidant activity. The identified chemical compounds
were correlated with the antioxidant properties, evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl
) rad ical scavenging assay (RSA), reducing power (RP), (β-carotene bleaching
inhibition (CBI) and lipid peroxidation inhibition by TBARS (thiobarbituric acid reactive
substances) assay (LPI). Carbohydrates were the most abundant macronutrients,
followed by proteins and ash. Fructose, mannitol and threalose were the predominant
sugars, but glucose was only found in B. edulis. Polyunsaturated fatty acids predominated over monounsaturated and saturated fatty acids. Palmitic, oleic and linoleic acids
were abundant in the three samples, but X. badius presented considerable amounts of
linoleic acid and less amounts of oleic acid. α- and β- tocopherols were quantified in all
the samples, but γ-tocopherol was only present in X. badius. The organic acids, oxalic
and fumaric, were quantified in the three samples; quinic acid was only present in L.
edodes, and malic and citric acids were only found in X. badius. p-Hydroxybenzoic, protocatechuic
and cinnamic acids were quantified in all the species, while p-coumaric acid
was only found in B. edulis. This species and X. badius revealed the highest antioxidant
properties, lowest EC50 values, which were related with the higher amounts in phenolic
compounds. B. edufis showed the highest RSA and RP (1.80±0.01, 0.63±0.02 mg/ml,
respectively), while X. badius presented the highest CBI and LPI (1.1 0±0.05, 0.37±0.02
mg/ml, respectively).
