Phenylethanoid glycosides in Veronica urticifolia extracts: characterization by HPLC-DAD-ESI/MS
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resumo
Veronica (Piantaginaceae) is a genus of 450 species found in temperate regions
of both hemispheres. ln this work, the aerial parts of Veronica urticifolia were
collected during flowering period from Mountain Goc in central Serbia.
Powdered samples (1 g) were extracted twice with methanol:water 80:20 (v/v).
After removing solvents, the combined extracts were re-dissolved in 20%
aqueous methanol, filtered through 0.22-1-lm disposable LC filter disks and
analyzed by high performance liquid chromatography coupled to a diode array
detector and electrospray-mass spectrometry (HPLC-DAD/ESI-MS). The phenolic
compounds were characterized according to their UV and mass spectra and
retention times. For the quantitative analysis, calibration curves of standard
compounds were used.
