Effect of electron beam irradiation doses in antioxidant activity and phenolic content of Portuguese chestnuts
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abstract
The objective of this study was to analyse the effect of
electron beam irradiation (0.5, I and 3 kGy) on the
antioxidant activity of Portuguese chestnuts (Castanea
saliva Mill.) using different in vitro assays, such as the 2,2-
diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging
activity, reducing power, inhibition of ~-carotene bleaching
and inhibition of lipid peroxidation using thiobarbituric
acid reactive species (TBARS). Total phenolics were
determined by spectrophotometric assays. Irradiated
samples seemed to preserve phenolics content and revealed
higher antioxidant activity than the control sample. The
most indicated dose to maintain antioxidants content, and to
increase reducing power and lipid peroxidation inhibition
was 1 kGy.
