The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020). Thanks to the Programa Apícola Nacional 2020‐2022 (National Beekeeping Program) for funding the project ‘Standardization of production procedures and quality parameters of bee products’. Thanks to the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project PDR2020‐1.0.1‐FEADER‐031734: ‘DivInA‐Diversification and Innovation on Beekeeping Production’ and project GreenHealth, Norte‐01‐0145‐FEDER‐000042. The authors acknowledge the National funding by FCT‐Foundation for Science and Technology, through the institutional scientific employment program‐contract with Soraia I. Falcão.
The exploitation of phenolic compounds in different fields has motivated researchers to explore eco-friendly and efficient extraction techniques. This study aimed to comparatively reveal that green extraction techniques (microwave- and ultrasound-assisted) are alternative to conventional extraction (maceration and magnetic stirring) with positive impact on the phenolic content, antioxidant activity, and bioactive profile of bee pollen extracts. The highest total phenolic and flavonoid content was reached using the microwave-assisted technique (MAE) with equivalent values of 28 and 8 mg g−1, with magnetic stirring and maceration showing a lower value. The composition profile of the extracts revealed the presence of twenty-six bioactive compounds, including thirteen phenolics and thirteen phenylamides. Although the extraction technique had little impact on the chemical diversity, the amount of bioactive compounds raised significantly with the use of the green extraction techniques, with gains between 40% and 60% for phenolics and up to 200% for phenylamides. The radical scavenging activity and the reducing power of the extracts confirmed that bee pollens are potent antioxidant source, with the most bioactive extracts corresponding to green extraction techniques. Consequently, all findings recommend the use of MAE as the technique most effective for the extraction of bioactive compounds from naturally encapsulated structures such as bee pollen.
