Development of an Electrochemical Aptasensor for the Detection of Human Osteopontin
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Overview
abstract
Electrochemical RNA aptasensor
To obtain bio-sensor with high affinity for rhOPN aiming to be used for cancer prognosis
Materials and methods
RNA aptamer details Isolated by SELEX process [1]
Sequence of the biotinylated RNA aptamer
5’-Biotin- CGGCCACAGAAUGAAAAACCUCAUCGAUGUUGCAUAGUUG-3’
Removal the interference of Rnase
Working RNA aptamer solution 4 nM in PBS.
RNA aptamer immobilization on a gold surfasse via streptavidin-biotin interaction
Electrochemical aptasensors, an emerging technology, enables the detection of protein biomarkers, which may be indicative of tumour activity. Osteopontin is a protein present in body fluids, being a possible biomarker since its overexpression has been related with breast cancer progression. An RNA aptamer, described in the literature, with affinity for human osteopontin, was synthetized, immobilized in a microelectrode gold surface and used for development electrochemical aptasensor for human OPN detection in standard solutions. Cyclic voltammetry results showed that this aptasensor allowed detecting human osteopontin with a detection limit of 8 nM, showing a satisfactory selectivity towards the target in the presence of others proteins, except for thrombin.
