Growth inhibitory activity of Coprinopsis atramentaria extract, phenolic acids and protected acetylated glucuronides on human tumor and non-tumor cell lines
Conference Paper
Overview
Overview
abstract
FCT for financial support to the Portuguese NMR network and
COMPETEIQRENIEU (PTDC/AGR-ALI/110062/2009, PEst-OE/AGR/UI0690/201 and PEstC/QUI/UI0686/2011)for financial support. S.A. Heleno (BD/7030412010) and R.C. Calhelha
(BPD/68344/2010) also thank FCT. POP/1-QREN and FSE.
Mushrooms represent an important source of compounds with antitumor and
immunostimulating properties. Our attention is being focused on the capacity of phenolic acids from mushrooms to miHb1t the growth of different tumor cell lines. Croprinopsis atramentaria (Bull.: Fr.) Redhead, Vilgalys & Moncalvo, is a wild edible mushroom previously characterized by us for its nutritional composition, and also showed a notable
antioxidant activity; p-hydroxybenzoic (4.71 mg/100 g dry weight), p -coumaric (0.82
mg/100 g) and cinnamic ( 1.70 mg/100 g) acids were identified in its phenolic
extract.PIJ·Icrcin, the growth inhibitory activity on human tumor cell lines (breast- MCF-7,
non-small cell lung- NCI-H460, colon- HCT- 15, hepatocellular- HepG2 and cervical HeLa) and non-tumor porcine liver primary cell culture (PLP2), was evaluated by sulforhodamine B assay, using C. atramentaria extract, phenolic acids, cinnamic acid and their protected glucuronides (HAGP, CoAGP and CAGP; Scheme) obtained reacting phydroxybenzoic (HA), p-coumaric (CoA) and cinnamic (CA) acids, respectively, with acetobromo-a-0-glucuronic acid methyl ester
