Firefighters’ biomonitoring: impact of fire combat on levels of urinary monohydroxyl metabolites of polycyclic aromatic hydrocarbons
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abstract
Firefighters, one of the most hazardous occupations, are regularly exposed to complex mixtures of
pollutants during fire combat. Polycyclic aromatic hydrocarbons (PAHs) are distributed between the
gaseous and particulate phases of air: they are one of the most health-relevant pollutants released
during fires because of their genotoxic, mutagenic, and carcinogenic properties [1]. This work aims to
assess the impact of firefighting activities on firefighters’ total exposure to PAHs.
Spot urine samples were collected in healthy and non-smoking firefighters before and after fire combat
activities. Six urinary PAH metabolites (1-hydroxynaphthalene (1OHNaph), 1-hydroxyacenaphthene
(1OHAce), 2-hydroxyfluorene (2OHFlu), 1-hydroxyphenanthrene (1OHPhen), 1-hydroxypyrene
(1OHPy), and 3-hydroxybenzo[a]pyrene (3OHB[a]P)) were quantified by liquid chromatography with
fluorescence detection [2-3].
Urinary 1OHNaph and 1OHAce were the predominant biomarkers of exposure in both non-exposed
and exposed firefighters, accounting with 63-98% of total levels of PAH biomarkers (ΣOH-PAHs).
2OHFlu, 1OHPhe, and 1OHPy contributed with 1-17%, 1-13%, and 0.3-10% of ΣOH-PAHs,
respectively. The PAH biomarker of carcinogenicity (3OHB[a]P) was not detected. Overall exposed
firefighters presented levels of ΣOH-PAHs that were 2-35% higher than for non-exposed subjects.
Urinary 2OHFlu seems to be the compound with the most pronounced increments in exposed
firefighters. Urinary 1OHPy levels were always lower than the benchmark of 0.5 μmol/mol creatinine
proposed by the American Conference of Governmental Industrial Hygienists. More studies assessing
PAH biomarkers of exposure but also biomarkers of effect and susceptibility are needed to evaluate
the impact of fire emissions on the health of firefighters.