Insights into olive mill wastewaters phenolic compounds
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abstract
Olive mill wastewaters (OMW) have high contents of phenolic compounds, which possess a
wide range of biological activities. Therefore, OMW are regarded as a potent source of biophenols
for food and pharmaceutical industries. Important biophenols occurring in OMW include tyrosol,
oleuropein and hydroxytyrosol, with the latter being commercially exploited as a novel ingredient
with a broad use in dietary supplements, functional foods and natural cosmetics [1 ,2].
Nevertheless, the phenolic profile of OMW is extremely complex and many compounds remain
unknown. The elucidation of new phenolic compounds in this matrix is an important task to
encourage the search of more bioactive compounds. Indeed, hydroxytyrosyl
acyclodihydroelenolate and p-coumaroyl-6' - secologanoside were recently identified in OMW
and proved to have greater antioxidant scavenging activity than hydroxytyrosol and oleuropein
[3].
The present study evaluates the recovery of some biophenols from three Portuguese OMW. For
that an ethyl acetate liquid-liquid extraction procedure, which has been previously optimized for
hydroxytyrosol extraction [4], has been used. On the other hand, this work also contributes to the
knowledge of OMW phenolic composition, through the elucidation of major phenolic constituents
in purified methanol (PME) or aqueous acetone extracts (PAAE).