Leccinum vulpinum Watling: nutritional, antioxidant and antitumour potential
Artigo de Conferência
Visão geral
Visão geral
resumo
FCT for the grant of F.S. Reis (SFRH/BD/111753/2015), D. Sousa
(SFRH/BD/98054/2013), and L. Barros (SFRH/BPD/107855/2015), and FCT and FEDER
for CIMO (UID/AGR/00690/2013) support
The global economic value of mushrooms is significant, not only due to their
value as food [1] but also their medicinal properties, some of them having
particularly been described to have antioxidant and antitumour potential [2,3].
Furthermore, some mushroom extracts have reached clinical trials. Additionally,
in Japan, PSK (a polysaccharide conjugate isolated from Trametes versicolor
(L.) Lloyd), is prescribed as an adjunct to conventional cancer therapies and
surgical treatments of colorectal and gastric cancers [4].
The aim of this work was to study the chemical profile of Leccinum vulpinum
Watling, regarding its content in nutrients and non-nutrients. Moreover, to study
the in vitro antioxidant and antitumour potential of its phenolic extract.
L. vulpinum wild samples were collected in Bragança, in the northeastern region
of Portugal. The nutritional value was assessed following standard procedures.
The individual profiles in soluble sugars, fatty acids and micronutrients were
obtained through chromatographic techniques. The antioxidant potential
of the phenolic extract was evaluated through the reducing power, DPPH
radical-scavenging activity and lipid peroxidation inhibition assays. To assess
the antitumour potential, the extract was screened against a panel of human
tumour cell lines (MCF-7, NCI-H460, HCT-15 and AGS). Studies of effect on cell
proliferation, cell cycle profile and apoptosis were performed on MCF-7 cells.
The effect on DNA damage was evaluated with the Comet assay. L. vulpinum showed a nutritional profile with low fat levels, fructose, mannitol
and trehalose as the foremost free sugars, and high percentage of mono- and
polyunsaturated fatty acids. The main isoforms of vitamin E detected were the
β- and γ-tocopherol. L. vulpinum also revealed the presence of phenolic acids
(e.g., gallic, protocatechuic and p-hydroxybenzoic acids) and other organic
acids (e.g., citric acid). The phenolic extract presented reducing capacity,
radical-scavenging activity and lipid peroxidation inhibition. Regarding the
antitumour potential, the extract inhibited the growth of all the tested cell lines.
In addition, it decreased cellular proliferation, induced apoptosis and caused
DNA damage of MCF-7 cells.
The present study highlights the importance of conserving the national
mycological resources, as a source of bioactive compounds with antioxidant
and antitumour activity.
