Chromatographic analysis of antioxidant and related compounds in Polyporus squamosus from different origins
Conference Paper
Overview
Overview
abstract
The antioxidant potential of mushrooms is mainly attributed to their composition
in polysaccharides, phenolic compounds, tocopherols and some organic acids [1].
Phenolic compounds contribute directly to the antioxidative action and play an
important role in stabilizing lipid peroxidation [2]; exhibit a wide range of bioactive
properties such as anti-allergenic, anti-inflammatory and antimicrobial, which have
been in part related to their antioxidant activity [3]. Tocopherols are important fatsoluble
antioxidants, acting in the cellular membrane; due to their role as scavenger
of free radicals protecting human cells against degenerative malfunctions [4]. Some
organic acids are very common in natural matrices; malic acid contributes to a
pleasantly sour taste and is often used as a food additive; citric acid is known due to
its antibacterial and antioxidant properties and fumaric acid is important because of its
antioxidant, anti-inflammatory, antimicrobial and acidifying properties [5]. The purpose
of the present study was to analyze antioxidant and related compounds (phenolic
compounds, tocopherols and organic acids) of Polyporus squamosus (Huds.) Fr.
samples originated from two different origins (Portugal and Serbia). Specimens of P.
squamosus were collected in Bragança (Northeast Portugal) and Jabučki rit (Northern
Serbia) during April 2015 and 2012, respectively. Phenolic compounds, organic acids
and tocopherols were determined by high performance liquid chromatograph (HPLC)
coupled to a diode array detector (DAD), in the two first cases, and a fluorescence
detector in the last one. With respect to phenolic and related compounds, p-hydroxybenzoic and cinnamic
acids were identified in both samples; the first one predominates in the sample from
Portugal, while cinnamic acid was more abundant in the sample from Serbia.
Tocopherols (α-, β and γ-isoforms) were found in the sample from Serbia, but in the
sample from Portugal, γ-tocopherol was not identified. This sample showed the
highest total tocopherols content, and revealed the highest level of β-tocopherol; γ-
tocopherol predominated in the sample from Serbia. Among organic acids, it was
possible to quantify oxalic, malic and fumaric acids in both samples. Malic acid was
found in higher amounts in the sample from Serbia.
Overall, the present study shows that mushroom samples from different origins
have dissimilar results, but are both rich in bioactive compounds, being a valuable
source for the development of natural medicines and nutraceuticals.
