Novel Incorporation of Red-Stage Haematococcus pluvialis Wet Paste as a Colourant and Enhancer of the Organoleptic and Functional Properties of Filloas ¿
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abstract
Haematococcus pluvialis Flotow is a microalga used as a nutraceutical, due to its high content
in bioactive compounds, mainly carotenoids, in which astaxanthin stands out [1]. Furthermore, H. plu-
vialis has shown a high antioxidant potential, and combined with its intense red colour, this microalga
could have dual functionality: as a colourant and a bioactive ingredient [ 2]. The process to obtain this
ingredient involves several transformation steps—namely, lyophilisation and saponification—raising
the costs to develop and obtain free astaxanthin, which paradoxically presents greater instability
and solubility than its esterified counterpart [ 3]. Thus, this study provides an alternative approach
for the application of red, astaxanthin-rich, H. pluvialis wet paste as a partial substitute for wheat
flour (7% and 13% w/w) in the preparation of filloas (Galician pancakes), a typical dessert from the
northwestern region of the Iberian Peninsula. To evaluate its power as a natural pigment, the stability
of colour over time (3, 6, and 9 days) was measured, and the results were compared with those of a
commercial colourant. At the same time, its physicochemical properties such as the microbiological
profile were measured to determine its functionality as a food preservative. As a result, redness
stability (a*) of 8% higher than that of the commercial colourant was obtained for the maximum
concentration of H. pluvialis analysed. The texture showed a significant response (p < 0.02), improving
its properties as the concentration of the microalga increased, which showed a tenacity of 3.23 N and
extensibility of 15.10 mm during the first 6 days, i.e., a 52% and 19% improvement, respectively, in
relation to the control group. In turn, an enrichment of carotenoids, fatty acids, and phenolic com-
pounds, in combination with a potential moderator of microbiological degradation by this unicellular
organism, gives added value to this food matrix.
This research was funded by project ED431 2020/06 (Galician Competitive Research Groups
Xunta de Galicia). This study was supported by project EQC2018-005011-P (Ministry of Science,
Innovation and Universities, Spain). All these programmes are co-funded by FEDER (EU). The
authors are also grateful to Foundation for Science and Technology (FCT, Portugal) for financial
support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); to FCT for S. Heleno
(CEECIND/03040/2017) and L. Barros contracts through the individual and institutional scientific
employment programme contract, respectively. This article is based upon work from the Sample
Preparation Study Group and Network, supported by the Division of Analytical Chemistry of the
European Chemical Society