Targeted metabolite analysis of aqueous extract of Rumex induratus leaves, in terms of phenolic
compounds and organic acids, and the study of its antioxidant activity against the DPPH• radical, a reactive
oxygen species, hypochlorous acid, and a reactive nitrogen species, nitric oxide (•NO), were performed.
The samples were collected in several locations, spontaneously occurring or from greenhouse culture, at
different stages of development and seasons. The phenolic composition was achieved by high-performance
liquid chromatography (HPLC)-diode array detection, and four hydroxycinnamic acid derivatives and 10
flavonoid glycosides (C- and O-heterosides) were determined. Organic acids composition was established
by HPLC-UV, revealing five compounds. The total amount of phenolic compounds and organic acids
were affected by growing conditions and developmental phase. The aqueous extract exhibited a doserelated
activity against all tested reactive species.
