Antioxidant and chealting activities of fermentation broths containing fructooligossacharides Conference Paper uri icon


  • Fructooligossacharides are present in plants and fruits at low concentrations and with varying individual relative proportions. So, for industrial application, fructooligossacharides extraction from natural sources may not be economically viable. Recently, several works have been published within this field of research usually aiming to establish the best experimental conditions to maximizing fructooligossacharides yield/synthesis [1-3]. However, although it is known that these compounds may have a great health impact, it is also reported that beneficial health effects may depend on the relative fructooligossacharides composition, seeming that nystose-rich diet is preferable compared to a kestoserich preparation, exhibiting a higher anti-hydroxyl radical activity [4,5]. So, in this work it is reported the results regarding a screening study concerning the bioactivity activity of the fermentation broth extracts obtained using fungi with potential for producing fructooligossacharides. The potential use of five fungi (i.e., A. aculeatus, A. japonicus, A. opinicosulum, P. thoumii and P. cornyphipum) was evaluated. Batch fermentation were carried out during 96 hours, at constant temperature (27 ºC) and agitation (100 rpm), being the initial sucrose concentration fixed at 30 g/L. Samples were taken at 24-h intervals and the radical scavenging activity as well as the iron binding ability of chelators, of the different broth extracts, were determined using the DPPH and ferrozine. The overall results obtained showed that although the extracts possessed relatively low bioactive activity; it was possible to set some preliminary insights that allowed selecting the most promissory(s) mold(s), which could enable achieving, in a near future, a final fructooligossacharides formulation with the greatest antioxidant capacity.
  • This work was financially supported by Project POCI-01-0145-FEDER-006984 – Associate Laboratory LSRE-LCM funded by FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e a Tecnologia.

publication date

  • January 1, 2016