Separation of nadolol racemates by high pH reversed-phase preparative fixed-bed chromatography: comparison of C18 materials
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abstract
Fixed-Bed technology will be used for the multicomponent
preparative separation of a pharmaceutical beta-blocker chiral drug.
New strategies using different achiral stationary phases will be
presented. Nadolol is a quaternary mixture of equal amounts of four
stereoisomers and will be used as case-study. A new methodology
for the design, optimization and experimental implementation of the
multicomponent separation will be introduced, including the use of
three different achiral adsorbents, the screening and choice of the
best adsorbent-solvent combination, taking in account the final
preparative separation using the fixed-bed technology. Extensive
experimental and simulation results will be presented, including
solvent screening, measurement of equilibrium adsorption isotherms,
breakthrough measurements, and fixed-bed (Azura prep HPLC unit)
experimental preparative separation using C18 columns under
reversed-phase mode.
“AIProcMat@N2020 - Advanced Industrial Processes and Materials for a Sustainable Northern Region of Portugal 2020”, with the reference NORTE-01-0145-FEDER-000006, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and of project POCI-01-0145-FEDER-006984 – Associate Laboratory LSRE-LCM funded by ERDF through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e Tecnologia.
