Irradiation and storage time effects on chemical parameters of processed samples of wild Macrolepiota procera (Scop.) Singer
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FCT and COMPETE/QREN/UE-strategic projects PEst-OE/AGR/UI0690/2011 (CIMO)
and PEst-C/EQB/LA0006/2011 (REQUIMTE); SFRH/BD/76019/2011 grant to A. Fernandes.
Macrolepiota procera (Scop.) Singer is one of the most popular mushrooms, being considered an
excellent edible species and highly appreciated for its nutritional and culinary values. Nevertheless,
due to its very fragile nature has to be processed to extend the shelf-life. Drying and freezing are
widely used postharvest technologies, but bacteria and mold can survive in these foods [1,2]. The
irradiation of mushrooms can be a safe method of decontamination, ensuring hygienic and sensory
quality [3]. The present work reports the effects of i) electron-beam irradiation (0.5, 1 and 6 kGy) and
storage period (0, 6 and 12 months) on the chemical composition of dried (at 30 ºC in an oven)
samples of wild Macrolepiota procera (Scop.) Singer; ii) gamma irradiation (0.5 and 1 kGy) on
organic acids and phenolic compounds of dried, frozen (at -20 ºC in a freezer) and fresh M. procera
samples. The fruiting bodies were collected in Trás-os-Montes (Northeast of Portugal) in November
2011. The electron-beam irradiation was performed at the Institute of Nuclear Chemistry and
Technology, in Warsaw, Poland. Gamma irradiation was performed in experimental equipment with
four 60Co sources at a dose rate of 2.3 kGy h-1. Proximate composition was evaluated by official
procedures, fatty acids were analysed by gas-chromatography coupled to flame ionization detection
(GC-FID), while sugars and tocopherols were determined by high performance liquid
chromatography (HPLC) coupled to refraction index (RI) and fluorescence detectors, respectively.
The organic acids and phenolic compounds were analysed by high performance liquid
chromatography coupled to a diode array detector (HPLC-DAD). The storage period had a higher
effect on all the evaluated parameters than the tested radiation doses, except for fatty acids, which
suffer significant changes with storage time and electron-beam irradiation. The differences caused by
processing type were also higher than the verified for irradiation dose. Furthermore, some effects of
the processing type (drying or freezing) were attenuated by irradiation: the lower amounts of oxalic
acid in fresh samples, malic acid in dried samples and citric acid in fresh and dried samples, was
significantly mitigated by irradiation treatment. Irradiation showed potential usefulness to be used
as complementary conservation technology since it attenuated some effects of dehydration and freeze
treatment.
