Evaluation of the bioactive properties of a secondary metabolite produced by Penicillium nordicum
Conference Paper
Overview
Overview
abstract
Fungi of the genus Penicillium are promising sources of bioactive substances. Fungal strains isolated
from poorly characterized habitais are currently under active investigation as potential producers of
biologically active compounds. Penicillium nordicum is mostly known by its ability to produce the
mycotoxin ochratoxin A in several protein- and salt-rich food matrices like processed meat and
cheese, but it can also produce an array of other secondary metabolites. In a previous study, strains
of this species were found to produce, under specific growth conditions, one unidentified compound in
apparently big amounts, without producing ochratoxin A.
The aim of the present study was to evaluate the bioactive properties of this compound in terms of
antioxidant, antimicrobial, cytotoxic and herbicidal properties. For that purpose, the fungus was grown
on ham-based médium and the aqueous extract of the compound was used for further tests.
The extract showed significant effects on seed germination as well as on root and shoot growth of
tomato, lettuce, wheat and oat. The root growth inhibition ranged from 74% to 98%, ana the shoot
growth was reduced in 23 to 88%, being tomato the most sensitive plant. The extract did not show
any cytotoxic activity in non-tumor porcine liver primary culture cells and human tumor cell lines. The
antibacterial effect on human clinicai isolates and antifungal effect on foodbome and environmental
isolates was also not significant.
Pestalotin was the predominant compound in the fungal extract, with questiomycin and 7-
hydroxypestalotin also being found at lower concentrations, as detected by Liquid Chromatography-
Mass Spectrometry (HPLC-MS/MS) based multi-metabolite method.
The extract was considered as a potential bioherbicide without noticeable negative effects on human
and animal cells and also on the environment. Further tests on herbicidal potential are now under
development.
The authors are grateful to the Foundatton for Science and Technology (FCT, Portugal) anü FEDER under
ProgrammePT2020 for financiai suppoit to CIMO (UID/AGR/00690/2013)
