Electrochemical aptasensor array for multiple detection of human osteopontin
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abstract
Most cancer diseases are associated with the presence of several biomarkers, as the proteins. The proteins,
when overexpressed in biological fluids could be used as poten:al diagnos:c or prognos:c markers
indica:ve of disease states [1,2]. Osteopon:n (OPN) is a phosphorylated glycoprotein and has been
reported as a poten:al breast cancer biomarker, its overexpression may be indica:ve of tumor forma:on,
cancer progression, metastasis and poor prognosis [3–6]. An early diagnosis of the disease is of utmost
importance to improve the pa:ent’s survival rate and therapeu:c efficacy, as well as to monitor the disease
recurrence. In this sense, it is essen:al to develop new, simple and cost-effec:ve methods holding a high
sensi:vity and reliability for the simultaneous detec:on of mul:ple protein disease biomarkers in biological
fluids [7]. The electrochemical mul:-aptasensors arrays has recently a\racted a\en:on for the detec:on of
various proteins disease biomarkers, due to their advantages, such as high sensi:vity, specificity, fast
detec:on, easier experimental procedures, lower cost prepara:on and the poten:al for miniaturiza:on, as
well as advantages of aptamers as bioreceptors elements such as high affinity and specificity towards a
specific target, easy to produce and synthesize, good stability and amenable to chemical modifica:on [8–
10]. This study describes the development of an electrochemical mul:-aptasensor array for the
simultaneous detec:on of human OPN using two specific aptamers. To enable mul:plexed protein assay,
the RNA and DNA aptamers were immobilized in the working electrodes of the dual-screen-printed gold
working electrodes (dual-SPGEs) via streptavidin-bio:n interac:on and using the [Fe(CN)6]3-/4- as the redox
probe for cyclic voltammetry (CV) measurements. The preliminary results herein report showed a good
response of the mul:-aptasensor array for the detec:on of human OPN. Moreover, the DNA/RNA mul:-
aptasensor array was able to selec:vely detect human OPN in the presence of other interfering proteins
such as thrombin, bovine serum albumin, bovine osteopon:n and lysozyme. Considering these preliminary
results, the mul:-aptasensor array holding the two aptamers could be a good alterna:ve for the specific
detec:on of human OPN and for cancer diagnosis overall.
